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rabbit anti grp94  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti grp94
    Rabbit Anti Grp94, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hsp90b1/gp96%2FHSP90B1%2FGRP94+Antibody+(9G10)/pmc13109171-68-27-32
    Average 94 stars, based on 9 article reviews
    rabbit anti grp94 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Metformin promotes antitumor immunity via endoplasmic reticulum-associated degradation of PD-L1
    Article Snippet: .. In antibody reaction buffer (PBS plus 1% BSA, 0.3% Triton X-100, pH 7.4), samples were stained with primary antibodies against active caspase 3 (9661L, 1:300; Cell Signaling Technology, Danvers, MA, USA ), CD8 (MCA609G, 1:100; BioRad, Hercules, CA, USA), GranzymeB (AF1865, 1:500; R&D Systems, Minneapolis, MN, USA), PD-L1 (13684, 1:200; Cell Signaling Technology), HSP90B1 (NBP2–42379, 1:300; Novus Biologicals, Littleton, CO, USA), GM130 (610823, 1:200; BD Biosciences, San Jose, CA, USA), Alexa fluor 488 Giantin (908701, 1:200; BioLegend, San Diego, CA, USA), TNG46 (NB110–62093, 1:400; Novus Biologicals) overnight at 4 °C, followed by Alexa 350, 488, 546, and 647 (1:3,000, Life Technologies) secondary antibodies at RT for 1 hr. .. Hoechst 33342 (Life Technologies) was used for nuclear staining.

    Article Title: The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer
    Article Snippet: To demonstrate the interaction between GRP78 and PD-L1, cells were stained using Duo-link proximity ligation assay (catalog DUO92101, Sigma-Aldrich) with primary antibodies to GRP78 (NBP1-06274; 1:100; Novus biologicals) and PD-L1 (66248-1-Ig; 1:100; Proteintech) per the manufacturer’s protocol. .. After proximity ligation assay, we stained cells with an antibody (nb300-619; 1:100; Novus Biologicals) to HSP90B1, which is an ER marker. .. Images were acquired using a Zeiss LSM710 laser microscope, as described above.

    other:

    Article Title: NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1
    Article Snippet: Commercial recombinant human GST (ab70456, Abcam), PD-L1/GST (ag12432; Proteintech), and NEK2-His (LS-G25896, LifeSpan Biosciences) were subjected to GST pull-down assay.

    Western Blot:

    Article Title: NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.
    Article Snippet: .. After trypsinization, samples were analyzed by Western blotting with primary antibodies against IRE1α (3294, Cell Signaling Technology), HSP90B1 (NBP2-42379, Novus Biologicals), EGFR (4267T, Cell Signaling Technology), NEK2 (24171-1-AP, Proteintech Group), and a-Tubulin (AF5012, Beyotime). ..

    Proximity Ligation Assay:

    Article Title: The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer
    Article Snippet: To demonstrate the interaction between GRP78 and PD-L1, cells were stained using Duo-link proximity ligation assay (catalog DUO92101, Sigma-Aldrich) with primary antibodies to GRP78 (NBP1-06274; 1:100; Novus biologicals) and PD-L1 (66248-1-Ig; 1:100; Proteintech) per the manufacturer’s protocol. .. After proximity ligation assay, we stained cells with an antibody (nb300-619; 1:100; Novus Biologicals) to HSP90B1, which is an ER marker. .. Images were acquired using a Zeiss LSM710 laser microscope, as described above.

    Marker:

    Article Title: The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer
    Article Snippet: To demonstrate the interaction between GRP78 and PD-L1, cells were stained using Duo-link proximity ligation assay (catalog DUO92101, Sigma-Aldrich) with primary antibodies to GRP78 (NBP1-06274; 1:100; Novus biologicals) and PD-L1 (66248-1-Ig; 1:100; Proteintech) per the manufacturer’s protocol. .. After proximity ligation assay, we stained cells with an antibody (nb300-619; 1:100; Novus Biologicals) to HSP90B1, which is an ER marker. .. Images were acquired using a Zeiss LSM710 laser microscope, as described above.



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